Last Thursday on February 16th, we observed cells undergoing mitosis on onion root tips. This was accomplished by a lengthy process that basically consisted of our instructor giving us "fixed" root tips, which is where cells are actively dividing. They are fixed because mitosis was suspended in the root apical meristem, or the root tip. We took these fixed root tips and put them in a dish labelled "Carnoy Fixative I" for 4 minutes. After 4 minutes, we transferred the root into a dish labelled "Carnoy Fixative II" for an additional 4 minutes. We then cut off about 1 to 2 mm of the root tip and put it on a slide. Once on the slide, we followed typical slide procedure and stained it with TBO. However, when we put the cover slip on the slide, we made sure to press straight down without twisting the cover slip. We then observed the slide under a compound microscope using oil immersion. This is what we saw: -Adalberto Marquez
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AuthorContent is created by students participating in the Plant Structure course at Oregon State University for Winter 2017. Archives
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