Last Tuesday on February 14th we went to the Library into a room on the 5th floor called the Bernard room. Here, we got into pairs to use one of the many computers in there that had a program called AISO. AISO is a program that's used to annotate images (like cross-sections of leafs) using ontology terms from the website Planteome.org. We used this time to annotate the pictures we had taken for our individual Natural History Projects, and if you ask me, there's no more romantic way to spend Valentine's Day than annotating pictures from our plants. Unfortunately for us, AISO is a fairly old program and has many bugs that makes it hard and frustrating to use. I wasn't able to annotate my pictures properly because of this, but luckily, I think it worked out. As you can see, I wasn't able to get AISO to properly annotate my image. The trichome came out relatively good, but the vascular bundle wasn't highlighted, although it was annotated. It's a good thing that it's easy to see as it's simply the big, dark spot in the middle. It was also hard to annotate the mesophyll but they're the white spots all throughout. I think a structure I should've annotated (but I didn't realize it was there due to time-constraint) was the palisade parenchyma cells on the top right. These elongated cells contain many chloroplasts and is the layer most responsible for photosynthesis. I showed the diagram of a dicot leaf for reference of what we're seeing in my images. It's good to note my image is slightly out of focus because my slice wasn't cut straight. Overall, this was a great experience and I wish a newer and updated version that fixed most of the bugs for AISO was released. It was a very fun lab nonetheless.
-Adalberto Marquez
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AuthorContent is created by students participating in the Plant Structure course at Oregon State University for Winter 2017. Archives
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